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日野 瑞城*; 浜田 信行*; 多鹿 友喜*; 舟山 知夫; 森村 吉博*; 坂下 哲哉; 横田 裕一郎; 深本 花菜*; 小林 泰彦; 依藤 宏*
Cell Structure and Function, 34(1), p.11 - 15, 2009/03
被引用回数:12 パーセンタイル:20.72(Cell Biology)Recently, SJL/J mice have been used as an animal model in studies of dysferlinopathy, a spectrum of muscle diseases caused by defects in dysferlin protein. In this study we irradiated muscle fibers isolated from skeletal muscle of SJL/J mice with heavy-ion microbeam, and the ultrastructural changes were observed by electron microscopy. The plasma membrane of heavy-ion beam irradiated areas showed irregular protrusions and invaginations. Disruption of sarcomeric structures and the enhancement of autophagy were also observed. In addition, many vesicles of varying size and shape were seen to be accumulated just beneath the plasma membrane. This finding further supports the recent hypothesis that dysferlin functions as a membrane fusion protein in the wound healing system of plasma membrane, and that the defect in dysferlin causes insufficient membrane fusion resulting in accumulation of vesicles.
日野 瑞城*; 和田 成一*; 多鹿 友喜*; 森村 吉博*; 浜田 信行*; 舟山 知夫; 坂下 哲哉; 柿崎 竹彦; 小林 泰彦; 依藤 宏*
Cell Structure and Function, 32(1), p.51 - 56, 2007/00
被引用回数:18 パーセンタイル:25.63(Cell Biology)単離骨格筋線維にマイクロビームにより重イオンを局所的に照射し、電子顕微鏡観察により超微形態変化を解析した。照射直後は細胞膜での不規則な形態の陥凹や突出,細胞内でのフィラメント配列の乱れが認められる一方で、時間経過に伴い多くの自食胞が観察された。このことから、重イオンは細胞の超微構造を変化させ、その回復には自家食が関与している可能性が示唆された。